7Y4I
Crystal structure of SPINDLY in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-11-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 127.735, 130.677, 141.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.340 - 2.850 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AF2D |
RMSD bond length | 0.003 |
RMSD bond angle | 0.613 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 65883 | 6457 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 13 | 13 |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 289 | 0.1 M bicine, 24% Polyethylene glycol 1,500, and 10% (v/v) 2-Propanol |