7Y4A

Crystal structure of human ELMO1 RBD-RhoG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-18
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	74.720, 63.750, 115.330
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	42.370 - 1.600
R-factor	0.198
Rwork	0.198
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jpp 4g0n
RMSD bond length	0.007
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.370	1.690
High resolution limit [Å]	1.600	1.600
Rmeas	0.048	0.541
Number of reflections	139984	22384
<I/σ(I)>	16.8	2.4
Completeness [%]	98.6
Redundancy	3.7
CC(1/2)	0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.91 M potassium phosphate dibasic and 0.49 M sodium phosphate monobasic monohydrate