7Y40

Crystal structure of a bright green fluorescent protein (StayGold) in jellyfish Cytaeis uchidae from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	P 61
Unit cell lengths	134.975, 134.975, 59.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.440 - 1.700
Rwork	0.178
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Af2 predicted
RMSD bond length	0.006
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.440	1.730
High resolution limit [Å]	1.700	1.700
Rmerge		0.556
Number of reflections	67651	3597
<I/σ(I)>	24.5
Completeness [%]	100.0
Redundancy	20.4
CC(1/2)	0.089

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M Ammonium sulfate, 0.1 M BisTris pH5.5, 25% PEG 3350