7Y3N
Crystal structure of SARS-CoV receptor binding domain in complex with human antibody BIOLS56
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.483, 159.018, 175.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.390 - 2.970 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg 4tsa |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.932 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.281 | 0.898 |
| Number of reflections | 64593 | 64593 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 291 | 20% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.9, 20% w/v Polyethylene glycol 4000 |
