7Y3M

Structure of SALL4 ZFC1 bound with 16 bp AT-rich dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	74.587, 65.706, 102.980
Unit cell angles	90.00, 106.78, 90.00

Refinement procedure

Resolution	33.789 - 2.723
R-factor	0.2266
Rwork	0.223
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7y3i
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.890	2.870
High resolution limit [Å]	2.720	2.720
Number of reflections	25181	25181
<I/σ(I)>	15.8
Completeness [%]	98.6
Redundancy	6.7
CC(1/2)	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M HEPES, pH 6.5, 10% polyethylene glycol 6000, 5% (v/v) 2-Methyl-2,4-pentanediol (MPD)