7Y3B
Crystal structure of TRIM7 bound to GN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 65 |
| Unit cell lengths | 79.768, 79.768, 52.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.660 - 1.760 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.18890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uma |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.660 | 1.824 |
| High resolution limit [Å] | 1.760 | 1.761 |
| Rmerge | 0.132 | 1.229 |
| Number of reflections | 18832 | 1870 |
| <I/σ(I)> | 20.08 | |
| Completeness [%] | 99.9 | |
| Redundancy | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000 |
