7Y3A
Crystal structure of TRIM7 bound to 2C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL17B |
| Synchrotron site | NFPSS |
| Beamline | BL17B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-09-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.228, 53.644, 80.999 |
| Unit cell angles | 90.00, 119.21, 90.00 |
Refinement procedure
| Resolution | 29.040 - 1.700 |
| R-factor | 0.1496 |
| Rwork | 0.148 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uma |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.700 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.046 | 0.140 |
| Number of reflections | 61973 | 3432 |
| <I/σ(I)> | 23.73 | |
| Completeness [%] | 99.4 | |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium formate pH 7.0 20%3350 0.2M NaCl |
