7Y18
Crystal structure of ribosomal ITS2 pre-rRNA processing complex from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.556, 116.142, 159.311 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 48.630 - 3.690 |
| R-factor | 0.2803 |
| Rwork | 0.278 |
| R-free | 0.31790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6of4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.157 | 0.585 |
| Number of reflections | 36773 | 2594 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2% tacsimate, pH 7.0, 0.1 M imidazole, pH 7.0, 2% 2-propanole, and 9% PEG 3350 |
