7Y11

Crystal structure of AtSFH5-Sec14 in complex with egg PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-09
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	99.976, 114.252, 112.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.050 - 1.950
R-factor	0.2008
Rwork	0.199
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aua
RMSD bond length	0.010
RMSD bond angle	1.529
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.171	2.021
Rpim	0.049	0.616
Number of reflections	47341	4662
<I/σ(I)>	16.2	1.45
Completeness [%]	99.8	99.9
Redundancy	13.1	11.6
CC(1/2)	0.997	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-Tris propone, pH 6.5, 20% PEG3350 and 0.2 M NaNO3