7Y0V

The co-crystal structure of BA.1-RBD with Fab-5549

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	64.770, 138.910, 178.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.090 - 2.480
R-factor	0.2148
Rwork	0.211
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e88
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.090	2.540
High resolution limit [Å]	2.480	2.480
Rmerge	0.104	1.668
Number of reflections	28861	2821
<I/σ(I)>	12.98
Completeness [%]	99.9	100
Redundancy	12.4	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	291	0.1 M HEPES sodium pH 7.25, 10% v/v 2-Propanol, 18% w/v Polyethylene glycol 4,000