7Y0L
SulE-S209A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.219, 139.863, 58.101 |
| Unit cell angles | 90.00, 101.60, 90.00 |
Refinement procedure
| Resolution | 36.070 - 1.290 |
| Rwork | 0.154 |
| R-free | 0.16720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q34 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.310 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rpim | 0.241 | |
| Number of reflections | 193359 | 9349 |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 96.7 | 93.4 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.993 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG4000 |
