7Y0B

Crystal structure of human short-chain acyl-CoA dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	80.994, 142.077, 154.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.170 - 2.076
R-factor	0.1973
Rwork	0.197
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vig
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.170	2.150
High resolution limit [Å]	2.076	2.076
Number of reflections	104537	8418
<I/σ(I)>	9.69
Completeness [%]	96.8
Redundancy	12.8
CC(1/2)	0.993	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	4% Tacsimate, 12% PEG3350, pH 5.0, 2 mM EMPA