7Y0A

Crystal structure of human short-chain acyl-CoA dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	78.894, 138.485, 150.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.493 - 2.317
R-factor	0.1958
Rwork	0.195
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vig
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.493	2.400
High resolution limit [Å]	2.317	2.317
Number of reflections	72068	6934
<I/σ(I)>	7.55
Completeness [%]	99.6
Redundancy	12.8
CC(1/2)	0.989	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	4% Tacsimate, 12% PEG3350, pH 5.0