7Y01

Crystal structure of ZmMCM10 in complex with 16nt ssDNA at 2.8. Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	85.180, 85.180, 103.912
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.880 - 2.800
R-factor	0.201
Rwork	0.199
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ebe
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.126	0.702
Number of reflections	11121	60687
<I/σ(I)>	14.6	2.1
Completeness [%]	99.5	98.6
Redundancy	5.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		293.15	0.2M lithium citrate, 20% PEG 3350