7XZG
High resolution crystal Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from Candida albicans complexed with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.643, 127.421, 87.855 |
| Unit cell angles | 90.00, 102.12, 90.00 |
Refinement procedure
| Resolution | 35.612 - 1.879 |
| R-factor | 0.1691 |
| Rwork | 0.169 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pym |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.612 | 1.947 |
| High resolution limit [Å] | 1.879 | 1.879 |
| Rmerge | 0.165 | 0.789 |
| Number of reflections | 124242 | 12367 |
| <I/σ(I)> | 10.24 | 2.12 |
| Completeness [%] | 99.3 | 99.28 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.991 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 32.5% v/v mPEG 550, 0.1M Trisodium citrate, pH 5.4, 1.0M sodium chloride, 20mM Tris |
