7XZA

Mutant (Y242F) of the N-terminal domain of fucoidan lyase FdlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2021-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	54.068, 58.150, 88.164
Unit cell angles	90.16, 101.69, 92.11

Refinement procedure

Resolution	27.750 - 2.080
R-factor	0.1757
Rwork	0.173
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xzf
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.000	2.190
High resolution limit [Å]	2.080	2.080
Rmeas	0.170	0.336
Number of reflections	57344	8676
<I/σ(I)>	3.9
Completeness [%]	91.0
Redundancy	1.7	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	291.15	0.1 M CAPS pH 10.5, 0.2 M lithium sulfate, 2 M ammonium sulfate