7XZ9

Mutant (E236A) of the N-terminal domain of fucoidan lyase FdlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2021-12-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	53.844, 58.005, 87.940
Unit cell angles	90.42, 101.60, 91.98

Refinement procedure

Resolution	34.810 - 1.540
R-factor	0.1648
Rwork	0.164
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xzf
RMSD bond length	0.006
RMSD bond angle	0.887
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.130	1.560
High resolution limit [Å]	1.540	1.540
Rmerge		0.164
Rmeas	0.204
Number of reflections	148952	4591
<I/σ(I)>	5.3
Completeness [%]	96.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.1 M Tris pH8.5, 0.2 M lithium sulfate, 30% (w/v) polyethylene glycol (PEG) 4000