7XZ1

TRIM E3 ubiquitin ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00933
Spacegroup name	I 41 2 2
Unit cell lengths	235.921, 235.921, 158.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.860 - 5.200
R-factor	0.2709
Rwork	0.266
R-free	0.31520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv2
RMSD bond length	0.002
RMSD bond angle	0.691
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	5.290
High resolution limit [Å]	5.200	5.200
Rmerge	0.135
Number of reflections	9007	452
<I/σ(I)>	49.84
Completeness [%]	99.9
Redundancy	28.8
CC(1/2)	0.992	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM Hepes-NaOH pH 7.5, 250 mM MgCl2, 37% (w/v) 5/4 PO/OH