7XYY

TRIM E3 ubiquitin ligase WT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-16
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	367.044, 98.064, 103.203
Unit cell angles	90.00, 101.32, 90.00

Refinement procedure

Resolution	49.030 - 7.100
R-factor	0.2742
Rwork	0.269
R-free	0.31370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv2
RMSD bond length	0.003
RMSD bond angle	0.772
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	7.220
High resolution limit [Å]	7.100	7.100
Number of reflections	5511	251
<I/σ(I)>	10.1
Completeness [%]	99.4
Redundancy	3.4
CC(1/2)	0.974	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.8 M NaCl, 8% (v/v) Ethanol