7XYX

Crystal structure of ZYG11B bound to CFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	52.777, 97.369, 122.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.400 - 2.870
R-factor	0.2049
Rwork	0.202
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep1
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	2.970
High resolution limit [Å]	2.870	2.870
Rmerge	0.259	1.507
Number of reflections	15037	1466
<I/σ(I)>	13.3
Completeness [%]	98.5
Redundancy	13.3
CC(1/2)		0.046

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M MES (pH 6.5), 1.6 M MgSO4