7XYW

Crystal structure of ZYG11B bound to AFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	52.880, 97.266, 122.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.490 - 2.500
R-factor	0.2285
Rwork	0.226
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep1
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.450	2.630
High resolution limit [Å]	2.500	2.500
Rmerge	0.109	1.413
Number of reflections	21062	3269
<I/σ(I)>	15.6
Completeness [%]	92.2
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES (pH 6.5), 1.6 M MgSO4