7XYT
Crystal structure of ZER1 bound to AFLH degron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-03 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.979, 138.273, 69.510 |
| Unit cell angles | 90.00, 117.38, 90.00 |
Refinement procedure
| Resolution | 34.610 - 1.500 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ep3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 138.270 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.118 | 1.568 |
| Number of reflections | 170861 | 12605 |
| <I/σ(I)> | 8.97 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M NH4F, 20% PEG3350 |
