7XXW

Macaca fascicularis galectin-10/Charcot-Leyden crystal protein with glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 65 2 2
Unit cell lengths	48.862, 48.862, 259.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.590 - 1.630
R-factor	0.1794
Rwork	0.177
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xrg
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.065	0.469
Number of reflections	24277	1145
<I/σ(I)>	26.5
Completeness [%]	99.9
Redundancy	17.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG