7XXN

HapR Quadruple mutant, bound to Qstatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-11
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	89.638, 43.795, 53.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.609 - 2.450
R-factor	0.2273
Rwork	0.224
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pbx
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.530	2.520
High resolution limit [Å]	2.450	2.450
Number of reflections	14926	786
<I/σ(I)>	8.3
Completeness [%]	99.0	99.7
Redundancy	8.4
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	7-8% PEG 8000, 100 mM CHES 9.5, 200mM NaCl