7XX6

Crystal Structure of Nucleosome-H1.0 Linker Histone Assembly (sticky-169a DNA fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	208.782, 102.370, 212.710
Unit cell angles	90.00, 101.12, 90.00

Refinement procedure

Resolution	48.830 - 3.390
R-factor	0.22884
Rwork	0.227
R-free	0.29611
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9 4qlc
RMSD bond length	0.008
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	3.570
High resolution limit [Å]	3.390	3.390
Number of reflections	122061	16714
<I/σ(I)>	10.5	1.1
Completeness [%]	98.9	93.4
Redundancy	6.7	6.7
CC(1/2)	1.000	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	40-45mM CaCl2, 25mM KCl, 10mM Na-acetate (pH 4.5)