7XX4
designed glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 65.716, 92.285, 191.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.370 - 2.430 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iya |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.954 | 2.486 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.086 | 1.070 |
| Rmeas | 0.094 | |
| Rpim | 0.038 | 0.437 |
| Number of reflections | 294658 | 4558 |
| <I/σ(I)> | 11.36 | |
| Completeness [%] | 99.6 | 99.91 |
| Redundancy | 6.36 | |
| CC(1/2) | 0.998 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | PEG 200, PEG 8000, Bis-Tris pronpane |
