7XVO

a bacteria protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 61
Unit cell lengths	127.210, 127.210, 44.384
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.800 - 2.000
R-factor	0.2322
Rwork	0.231
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jdv
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.600	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	28150	2777
<I/σ(I)>	14.39
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	2-Propanol, Sodium citrate tribasic dihydrate, Polyethylene glycol 400