7XVN

Structural basis for DNA recognition feature of retinoid-related orphan receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	33.627, 64.584, 238.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 2.302
R-factor	0.2117
Rwork	0.208
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l6l
RMSD bond length	0.007
RMSD bond angle	0.672
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.384
High resolution limit [Å]	2.302	2.302
Rmerge	0.142
Number of reflections	43676	2370
<I/σ(I)>	11.33
Completeness [%]	99.0
Redundancy	7.1
CC(1/2)	0.995	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	0.1 M MES pH 5.6, 0.1 M (NH4)2SO4, 0.01 M MgCl2, 22% PEG 8K