7XVM
Crystal Structure of Nucleosome-H5 Linker Histone Assembly (sticky-169a DNA fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 98.15 |
Detector technology | PIXEL |
Collection date | 2017-09-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.160, 103.758, 212.955 |
Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.030 | 2.990 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 105157 | 14236 |
<I/σ(I)> | 13.7 | 1 |
Completeness [%] | 98.8 | 92.4 |
Redundancy | 6.6 | 6.1 |
CC(1/2) | 0.999 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 40-45mM CaCl2, 25mM KCl, 10mM Na-acetate (pH 4.5) |