7XVA
Crystal structure of the Human TR4 Ligand Binding Domain in complex with the JAZF1 corepressor fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-07 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 76.851, 76.851, 98.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.090 - 1.856 |
Rwork | 0.197 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p0u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.856 | 1.856 |
Number of reflections | 25132 | 22758 |
<I/σ(I)> | 36 | |
Completeness [%] | 98.0 | |
Redundancy | 9.5 | |
CC(1/2) | 0.998 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | HEPES, ammonium sulfate, sodium acetate, MPD |