7XV9

Crystal structure of the Human TR4 DNA-Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	32.229, 32.229, 128.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.031 - 1.599
Rwork	0.176
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dzu
RMSD bond length	0.013
RMSD bond angle	1.761
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.599	1.599
Number of reflections	17222	15147
<I/σ(I)>	21.2
Completeness [%]	99.6
Redundancy	9.1
CC(1/2)	0.996	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Malonate