7XV7

Crystal structure of ZYG11B bound to ORF10 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	52.504, 96.854, 121.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.210 - 2.600
R-factor	0.2057
Rwork	0.203
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep1
RMSD bond length	0.008
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.260	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.056	0.968
Number of reflections	19576	1910
<I/σ(I)>	31.83
Completeness [%]	98.1
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH 6.5, 1.6 M MgSO4.