7XUX

Crystal structure of a FIC domain containnig protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979813
Spacegroup name	C 2 2 2
Unit cell lengths	115.464, 282.767, 73.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.860 - 2.780
R-factor	0.231
Rwork	0.229
R-free	0.25740
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.380	2.850
High resolution limit [Å]	2.780	2.780
Rmerge	0.206	4.060
Number of reflections	406690	30176
<I/σ(I)>	0.8
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M MES pH 6.5, 25% Glycerol