7XUV

Crystal structure of RPA70N-RMI1 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	40.930, 50.263, 52.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.240 - 1.600
R-factor	0.1884
Rwork	0.187
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.240	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.069	0.342
Rpim	0.032	0.158
Number of reflections	22637	2779
<I/σ(I)>	13.8
Completeness [%]	89.4
Redundancy	4.6
CC(1/2)	0.997	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	278	30% PEG 2000 MME