7XUT

Crystal structure of RPA70N-WRN fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	32.936, 58.609, 111.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.590 - 1.600
R-factor	0.181
Rwork	0.179
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.590	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.097	0.152
Rpim	0.042	0.064
Number of reflections	54591	2870
<I/σ(I)>	13.82
Completeness [%]	99.8	100
Redundancy	6.2
CC(1/2)	0.992	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	20% PEG3350, 200 mM ammonium sulfate