7XSW
Structure of SARS-CoV-2 antibody S309 with GX/P2V/2017 RBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.639, 73.599, 105.615 |
| Unit cell angles | 110.53, 92.08, 97.05 |
Refinement procedure
| Resolution | 48.540 - 3.300 |
| R-factor | 0.2808 |
| Rwork | 0.277 |
| R-free | 0.32160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7r6x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.543 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 3.421 | 3.303 |
| Number of reflections | 40975 | 6611 |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 89.0 | |
| Redundancy | 4.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M magnesium chloride hexahydrate, 0.1M MES (pH 6.0), 20% w/v PEG 6000 |
