7XR7

Drimenyl diphosphate synthase D303E from Streptomyces showdoensis in complex with farnesyl diphosphate (FPP) and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-15
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	51.640, 114.210, 180.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.430 - 1.630
R-factor	0.1887
Rwork	0.188
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xqz
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.100	57.100	1.660
High resolution limit [Å]	1.630	8.930	1.630
Rmerge	0.077	0.038	0.617
Rmeas	0.091	0.052	0.725
Rpim	0.049	0.036	0.375
Total number of observations	218692	718	11379
Number of reflections	66036	367	3279
<I/σ(I)>	11.1	25.2	2.1
Completeness [%]	98.8	81.2	99.8
Redundancy	3.3	2	3.5
CC(1/2)	0.993	0.941	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	HEPES, NaCl