7XQY

Crystal structure of T2R-TTL-15 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	105.420, 158.330, 180.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.580 - 2.350
R-factor	0.1998
Rwork	0.199
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.580	41.580	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.105	0.053	1.202
Rmeas	0.113	0.058	1.286
Rpim	0.041	0.022	0.454
Total number of observations	949375	10089	72091
Number of reflections	126288	1532	9214
<I/σ(I)>	11.8	28.1	1.7
Completeness [%]	99.9	97.5	100
Redundancy	7.5	6.6	7.8
CC(1/2)	0.997	0.996	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	6% PEG, 5% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7