7XPW
Structure of OhTRP14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.772, 44.912, 38.491 |
| Unit cell angles | 90.00, 115.10, 90.00 |
Refinement procedure
| Resolution | 34.860 - 1.770 |
| R-factor | 0.1718 |
| Rwork | 0.168 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wou |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.602 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.052 | 0.181 |
| Number of reflections | 10429 | 10429 |
| <I/σ(I)> | 28.535 | |
| Completeness [%] | 98.4 | |
| Redundancy | 6.3 | 6.1 |
| CC(1/2) | 0.966 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 289 | 30% PEG 3350, 0.1M Bis-tris PH 5.9 |
