Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.578, 104.578, 322.501 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.020 - 2.850 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ey4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.020 | 2.940 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.150 | 0.566 |
| Number of reflections | 48576 | 4372 |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.988 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 100 mM Tris HCl buffer pH 9.0, 20 % PEG 3350, 200 mM KNO3 |
