7XMP
High resolution structure of lectin-like Ox-LDL receptor 1 in space group P 32 2 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.030, 50.030, 91.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.330 - 1.270 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ypu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS (20210205) |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.330 | 43.327 | 1.280 |
| High resolution limit [Å] | 1.257 | 3.523 | 1.257 |
| Rmerge | 0.098 | 0.071 | 1.641 |
| Rmeas | 0.101 | 0.074 | 1.692 |
| Rpim | 0.025 | 0.018 | 0.405 |
| Total number of observations | 26662 | 30995 | |
| Number of reflections | 36626 | 1832 | 1833 |
| <I/σ(I)> | 15.4 | 32.2 | 1.8 |
| Completeness [%] | 99.5 | 99.8 | 96.6 |
| Redundancy | 16.7 | 14.6 | 16.9 |
| CC(1/2) | 0.998 | 0.997 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 2M ammonium sulfate, 0.2 M sodium chloride, 0.1M sodium cacodylate |
