7XLJ

The crystal structure of ORE1(ANAC092) NAC domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	70.333, 73.761, 78.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.880 - 2.450
R-factor	0.2192
Rwork	0.217
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ulx
RMSD bond length	0.009
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.880	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.048	0.488
Number of reflections	15181	1301
<I/σ(I)>	26.58	1.77
Completeness [%]	98.5	86.1
Redundancy	6.9	4.9
CC(1/2)	0.998	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M tris pH 8.5, 7% PEG20K