7XKC

Crystal structure of Daucus Carrot hypoglycemic peptide (DCHP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	53.850, 66.210, 121.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.780 - 2.560
R-factor	0.2463
Rwork	0.241
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rdy
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.780	2.630
High resolution limit [Å]	2.560	2.560
Rmerge	0.200	0.605
Number of reflections	14451	955
<I/σ(I)>	14.8	2.4
Completeness [%]	99.1	93.5
Redundancy	9.5
CC(1/2)	0.975	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Sodium sulfate decahydrate, 20% (w/v) Polyethylene glycol (PEG) 3350