7XJE
Crystal structure of bacteriorhodopsin in the K state refined against the extrapolated dataset
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 15 |
| Detector technology | PIXEL |
| Collection date | 2020-10-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.570, 60.570, 110.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.330 |
| Rwork | 0.274 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1300028945 |
| Phasing software | CNS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 290824 | 44229 |
| <I/σ(I)> | 10.08 | 1.24 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | MO, 2.0-2.5 M PHOSPHATE |
