7XIV
Structural insight into the interactions between Lloviu virus VP30 and nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 64.251, 64.251, 175.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.126 - 2.498 |
R-factor | 0.233 |
Rwork | 0.229 |
R-free | 0.26940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dvw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.298 |
Data reduction software | MOSFLM |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.126 | 2.598 |
High resolution limit [Å] | 2.498 | 2.498 |
Rpim | 0.020 | 0.084 |
Number of reflections | 13571 | 660 |
<I/σ(I)> | 40.4 | |
Completeness [%] | 100.0 | |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris-HCl, pH 7.0, 0.25 M NaCl, 22% (w/v) polyethylene glycol 3350 |