7XHR

Crystal structure of Wild Type Cypovirus Polyhedra produced by cell-free protein synthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	I 2 3
Unit cell lengths	103.610, 103.610, 103.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.299 - 1.801
Rwork	0.149
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gqm
RMSD bond length	0.009
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.800	1.800
Number of reflections	17297	414
<I/σ(I)>	5.5	1.53
Completeness [%]	100.0
Redundancy	399
CC(1/2)	0.990	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization