7XGW

Apo structure of LW domain from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 32 2 1
Unit cell lengths	111.724, 111.724, 93.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.265 - 2.250
R-factor	0.2025
Rwork	0.199
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fax
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.101	1.111
Number of reflections	32354	2857
<I/σ(I)>	19.5
Completeness [%]	99.4
Redundancy	19.1
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	1.5M Lithium sulfate monohydrate, 0.1M Tris pH 8.5