7XGC

Crystal structure of influenza polymerase acidic subunit N-terminal domain crystallized by ammonium sulfate with glycan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	66.177, 66.177, 125.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.536 - 1.600
R-factor	0.2063
Rwork	0.205
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zqq
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	MOLREP
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.047	1.174
Rmeas	0.049	1.282
Rpim	0.014	0.495
Number of reflections	37623	1755
<I/σ(I)>	23
Completeness [%]	99.7	96.4
Redundancy	11.8	6.2
CC(1/2)	1.000	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	291	0.1M MES, 1.275M Ammonium Sulfate, 0.1M POTASSIUM CHLORIDE, 9%(v/v) TREHALOSE