7XGB

Crystal structure of the ctcP from Streptomyces aureofaciens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	P 2 21 21
Unit cell lengths	116.393, 121.281, 179.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.700
R-factor	0.1777
Rwork	0.176
R-free	0.22430
Structure solution method	SAD
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.177	0.082	0.812
Total number of observations	1038277
Number of reflections	70271	7381	6909
<I/σ(I)>	8.1
Completeness [%]	100.0	100	100
Redundancy	14.8	14	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M BIS-TRIS pH 6.0, 0.2M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3350