7XF1
Crystal strucutre of apoCasDinG in complex with ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.181, 111.181, 152.736 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.460 - 3.200 |
R-factor | 0.2089 |
Rwork | 0.207 |
R-free | 0.23550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fwr |
Data scaling software | HKL-3000 |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 18489 | 1797 |
<I/σ(I)> | 7 | |
Completeness [%] | 99.8 | |
Redundancy | 18 | |
CC(1/2) | 0.984 | 0.575 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1M MES pH 6.0, 1.6M Ammonium sulfate |